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Mapk1 Antibody

Mapk1 Antibody

Product Specifications

UniProt

P63086

Reactivity

Frog, Human, Mouse, Rat

Immunogen

ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.

Target

Mapk1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Affinity Purified

Concentration

Batch dependent

Form

Liquid

Molecular Weight

42/44

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB) . Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Tested Applications

IHC, WB

NCBI Accession Number

P63086

Host or Source

Rabbit

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