Gria1 Antibody
Gria1 Antibody
Product Specifications
UniProt
P19490
Reactivity
Human, Mouse, Rat
Immunogen
GluR1 (Ser845) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser845 of rat GluR1.
Target
Gria1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Affinity Purified
Concentration
Batch dependent
Form
Liquid
Molecular Weight
100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB) . Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.
Tested Applications
IF, IHC, WB
NCBI Accession Number
P19490
Host or Source
Rabbit
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