SingleFlowEx CD117 Alexa Fluor® 700

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

104D2

Applications

FC (QC tested)

Concentration

0.050 mg/mL

Format

Alexa Fluor® 700

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Stevenson KS, Mc Glynn L, Hodge M, Mc Linden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Mar 27., URL: https://www.ncbi.nlm.nih.gov/pubmed/19326970?ordin, *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL: , *Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110 (1) :151-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/17379743?ordin, *Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct;30 (10) :1241-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16387359?ordin, *Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117) . Exp Hematol. 2000 Jun;28 (6) :660-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/10880752?ordin, *Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49 (2) :97-110., URL: https://www.ncbi.nlm.nih.gov/pubmed/10889948?ordin, *Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c-Kit. Blood. 1999 Sep 15;94 (6) :1979-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/10477727?ordin, *Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998 Feb 1;91 (3) :898-906., URL: https://www.ncbi.nlm.nih.gov/pubmed/9446650?ordina, *Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1;90 (1) :111-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/9207445?ordina, *Use of this product has not been published., URL: