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NANOG Antibody (Biotin)

NANOG Antibody (Biotin)

Product Specifications

Product Name Alternative

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

UniProt

Q9H9S0

Reactivity

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Human Nanog specific antibody was purified by affinity chromatography and then biotinylated.

Target

NANOG

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Stem Cell & Developmental Biology

Purification

Nanog antibody was purified by affinity chromatography and then biotinylated.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA:Sandwich:To detect hNanog by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. Western Blot:To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

Q9H9S0

Host or Source

Rabbit

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