CD22 Antibody
CD22 Antibody
Product Specifications
Product Name Alternative
SIGLEC2, SIGLEC-2
UniProt
Q0EAF5
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCD22. Human CD22 specific antibody was purified by affinity chromatography employing immobilized hCD22 matrix.
Target
CD22
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
CD22 antibody was purified by affinity chromatography employing immobilized CD22 matrix.
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Indirect:To detect hCD22 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Sandwich:To detect hCD22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot:To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
Q0EAF5
Host or Source
Rabbit
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog