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CD22 Antibody

CD22 Antibody

Product Specifications

Product Name Alternative

SIGLEC2, SIGLEC-2

UniProt

Q0EAF5

Reactivity

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCD22. Human CD22 specific antibody was purified by affinity chromatography employing immobilized hCD22 matrix.

Target

CD22

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

CD22 antibody was purified by affinity chromatography employing immobilized CD22 matrix.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA:Indirect:To detect hCD22 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Sandwich:To detect hCD22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot:To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

Q0EAF5

Host or Source

Rabbit

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