Il15 Antibody

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-15. Murine IL-15 specific antibody was purified by affinity chromatography employing immobilized mIL-15 matrix.

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-15 (5.0 ng/mL), a concentration of 0.4 - 0.5 μg/mL of this antibody is required.ELISA:Indirect:To detect mIL-15 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.SandwichTo detect mIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-15 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15. Western Blot:To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.