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Il15 Antibody (Biotin)

Il15 Antibody (Biotin)

Product Specifications

Product Name Alternative

IL-15, AI503618Interleukin-15IL-15

UniProt

P48346

Reactivity

Mouse

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-15. Murine IL-15 specific antibody was purified by affinity chromatography and then biotinylated.

Target

Il15

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA:Sandwich:To detect mIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine IL-15 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15. Western Blot:To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

P48346

Host or Source

Rabbit

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