IL1RN Antibody (Biotin)

Produced from sera of rabbits immunized with highly pure recombinant Human IL-1RA. Human IL-1RA specific antibody was purified by affinity chromatography and then biotinylated.

ELISA:Sandwich:To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-1RA as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA. Western Blot:To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.