SERPINE1 Antibody

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1 (human Plasminogen Activator Inhibitor-1) . Human PAI-1 specific antibody was purified by affinity chromatography employing immobilized hPAI-1 matrix.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hPAI-1 (4.5 μg/mL), a concentration of at least 15.0 μg/mL of this antibody is required.ELISA:Indirect:To detect hPAI-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1.SandwichTo detect hPAI-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human PAI-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1. Western Blot:To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.