IL6 Antibody (Biotin)
IL6 Antibody (Biotin)
Product Specifications
Product Name Alternative
HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell stimulatory factor 2
UniProt
P05231
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-6. Human IL-6 specific antibody was purified by affinity chromatography and then biotinylated.
Target
IL6
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Sandwich:To detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-6 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot:To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P05231
Host or Source
Rabbit
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