Il2 Antibody
Il2 Antibody
Product Specifications
Product Name Alternative
Il-2, Interleukin-2, T-cell growth factor, IL-2
UniProt
P17108
Reactivity
Rat
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Rat IL-2 specific antibody was purified by affinity chromatography employing immobilized Rat IL-2 matrix.
Target
Il2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-2 (1.0 ng/mL), a concentration of ELISA:Indirect:To detect Rat IL-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.SandwichTo detect Rat IL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat IL-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2. Western Blot:To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, NeA, WB
NCBI Accession Number
P17108
Host or Source
Rabbit
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