What is the catalog number for Vegfa Antibody?

The catalog number for Vegfa Antibody is orb1272689.

What sizes are available for Vegfa Antibody?

Vegfa Antibody is available in the following size(s): 0.1 mg.

Is Vegfa Antibody in stock?

Vegfa Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

Vegfa Antibody

Product Specifications

Product Name Alternative

VPF, Vegf, VEGF-A, VEGF164, Vascular endothelial growth factor A, Vascular permeability factor

UniProt

P16612

Reactivity

Rat

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor) . Rat VEGF specific antibody was purified by affinity chromatography employing immobilized Rat VEGF matrix.

Target

Vegfa

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cardiovascular Research; Cell Biology

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Rat VEGF (10 ng/mL), a concentration of Indirect:To detect Rat VEGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.SandwichTo detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat VEGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF. Western Blot:To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.