CCL26 Antibody

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot:To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.