NGF Antibody
NGF Antibody
Product Specifications
Product Name Alternative
Nerve growth factor, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428 , Beta-nerve growth factorBeta-NGF
UniProt
P01138
Reactivity
Human
Immunogen
Beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.
Target
NGF
Clonality
Monoclonal
Clone
M85-2B
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human β-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western Blot:To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Tested Applications
ELISA, NeA, WB
NCBI Accession Number
P01138
Host or Source
Mouse
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