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BDNF Antibody

BDNF Antibody

Product Specifications

Product Name Alternative

ANON2, BULN2Brain-derived neurotrophic factor, Abrineurin, BDNF

UniProt

P23560

Reactivity

Human, Mouse, Rat

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human Brain-Derived Neurotrophic Factor) .

Target

BDNF

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Anti-hBDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA:To detect hBDNF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBDNF. Western Blot:To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

P23560

Host or Source

Rabbit

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