BDNF Antibody
BDNF Antibody
Product Specifications
Product Name Alternative
ANON2, BULN2Brain-derived neurotrophic factor, Abrineurin, BDNF
UniProt
P23560
Reactivity
Human, Mouse, Rat
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human Brain-Derived Neurotrophic Factor) .
Target
BDNF
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Anti-hBDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:To detect hBDNF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBDNF. Western Blot:To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P23560
Host or Source
Rabbit
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