TNFSF10 Antibody

Product Specifications

Product Name Alternative

APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2, CD253

UniProt

P50591

Reactivity

Human

Immunogen

The monoclonal antibody was produced from a hybridoma using purified recombinant, insect cell derived, hTGFβ1.

Target

TNFSF10

Clonality

Monoclonal

Clone

M66-4B

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

This IgG1k antibody was purified on a Protein G affinity column.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human TGFβ1 when used in conjunction with compatible secondary reagents. Western Blot:To detect hTGFβ1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFβ1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with human TGFβ2 and TGFβ3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.