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CCL7 Antibody

CCL7 Antibody

Product Specifications

Product Name Alternative

Monocyte chemotactic protein 3, MCP3, SCYA6, SCYA7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, MCP-3, FIC, MARC, NC28

UniProt

P80098

Reactivity

Human

Immunogen

The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMCP-3 as the immunizing antigen.

Target

CCL7

Clonality

Monoclonal

Clone

PEPY042_hMCP3

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100ng/mL), a concentration of 1 - 3 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect recombinant human MCP-3 when used with our biotinylated antigen affinity purified anti-human MCP-3 (XP-5221Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Tested Applications

ELISA, NeA, WB

NCBI Accession Number

P80098

Host or Source

Mouse

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