Anti-Atg2A Antibody

Rabbit Polyclonal Antibody specific to Atg2A

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Atg2A Rabbit Polyclonal Antibody

Gene Name

ATG2A

NCBI Gene ID

UniProt

Q2TAZ0

Cellular Locus

Preautophagosomal structure membrane, Lipid droplet, Endoplasmic reticulum membrane

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids at the C-terminus of human ATG2A.

Target Antigen

Autophagy-related protein 2 homolog A

Target

Atg2A

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Autophagy

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Lipid transfer protein involved in autophagosome assembly (PubMed:28561066, PubMed:31271352, PubMed:30952800). Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (PubMed:31271352, PubMed:30952800). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PubMed:31271352, PubMed:30952800). Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352). Also regulates lipid droplets morphology and distribution within the cell (PubMed:22219374, PubMed:28561066). {PubMed:22219374, PubMed:28561066, PubMed:30952800, PubMed:31271352}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~213kDa is detected. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human Atg2A.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Two human Atg2 homologs (Atg2A, Atg2B) are critical for autophagosome formation as silencing of both results in the accumulation of unclosed autophagic structures. Starvation-induced autophagy targets Atg2A to the initiation site of autophagosome biogenesis, where it associates with DFCP1, WIPI-1, and other autophagy- related proteins. Atg2 proteins also function in lipid droplet metabolism as depletion of both Atg2A and AtgB results in changes in the size, number, and distribution of lipid droplets. An increase in Atg2A expression during etoposide- and doxorubicin-induced apoptosis suggests that Atg2A may be a useful indicator of topoisomerase II inhibitor-mediated apoptosis.