Anti-Nitrotyrosine Antibody
Mouse Monoclonal Antibody specific to Nitrotyrosine
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-Nitrotyrosine Mouse Monoclonal Antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Canine
Immunogen
3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA.
Target
Nitrotyrosine
Clonality
Monoclonal
Isotype
IgG2a
Type
Antibody
Applications
WB, IHC, ICC/IF, IP, ELISA, FCM, AM
Field of Research
Neuroscience
Purification Method
Purified by Protein G affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at 1.0ug/mL.
<br><br>Positive control: Linsidomine (SIN-1)-treated BSA.
<br><br>Immunohistochemistry: use at 1-5ug/mL.
<br><br>These are recommended concentrations.
<br><br>Endusers should determine optimal concentrations for their applications.
Storage Conditions
This antibody is stable for at least one (1) year at -20°C.
Specificity
This antibody recognizes 3-nitrotyrosine moieties, species-independent. No detectable reactivity with non-nitrated tyrosine.
Formulation
PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide.
Buffer pH
pH 7.4
Target Background
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P- 450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, its plasma concentration may be a useful determinant of NO- dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.
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