Anti-Nitrotyrosine Antibody

Mouse Monoclonal Antibody specific to Nitrotyrosine

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Nitrotyrosine Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Human, Mouse, Rat, Canine

Immunogen

3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA.

Target

Nitrotyrosine

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, ELISA, FCM, AM

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1.0ug/mL. Positive control: Linsidomine (SIN-1)-treated BSA. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes 3-nitrotyrosine moieties, species-independent. No detectable reactivity with non-nitrated tyrosine.

Formulation

PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P- 450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, its plasma concentration may be a useful determinant of NO- dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.