3-Indoxyl phosphate (sodium)
3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
Product Specifications
CAS Number
[3318-43-2]
Product Name Alternative
3-IP (sodium)
UNSPSC
12352005
Hazard Statement
H302-H315-H319-H335
Target
Phosphatase
Type
Reference compound
Related Pathways
Metabolic Enzyme/Protease
Applications
Metabolism-sugar/lipid metabolism
Field of Research
Metabolic Disease
Assay Protocol
https://www.medchemexpress.com/3-indoxyl-phosphate-sodium.html
Smiles
O=P(OC1=CNC2=C1C=CC=C2)(O[Na])O[Na]
Molecular Formula
C8H6NNa2O4P
Molecular Weight
257.09
Precautions
P261-P264-P270-P271-P280-P302+P352-P304+P340-P330-P362+P364-P405-P501
References & Citations
[1]Tsou, et al. A new colorimetric method for the determination of alkaline phosphatase with indoxyl phosphate. Anal. Biochem. 11 (1), 54-64 (1965) .
Shipping Conditions
Room temperature
Scientific Category
Reference compound1
Clinical Information
No Development Reported
Available Sizes
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