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ScFv control Protein, Fc Tag (MALS verified)

Anti-DNP-scFv, Fc Tag (DNP-H5254) is expressed from human 293 cells (HEK293) . It mimics the scFv presented on Anti-DNP.

Product Specifications

Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens) . Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.8 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Host

HEK293

Target

ScFv control

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind scFv control Protein, Fc Tag (Cat. No. DNP-H5254) with a linear range of 0.1-1 ng/mL (QC tested).

Format

Liquid

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

52.8 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

100mg*1

Host or Source

HEK293

Species

Human

Preservative

Trehalose

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