TJP1 antibody
Product Specifications
Background
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090) . ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172) . It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867) . ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs) . It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886) .
Synonyms
Tight junction protein ZO-1|Tight junction protein 1|Zona occludens protein 1|Zonula occludens protein 1|TJP1|ZO1
Gene ID
7082
UniProt
Q07157
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Tight junction protein 1 (zona occludens 1)
Target
TJP1
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, FC, IF, IP
Field of Research
Neuroscience
Purification
Immunogen affinity purified
Dilution
WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
230 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, FC, IF, IP
Immunogen Sequence
480-742aa
Curated Selection
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