VIM antibody
Product Specifications
Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Synonyms
Vimentin|VIM
UniProt
P08670
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
vimentin
Target
VIM
Clonality
Monoclonal
Isotype
IgG1
Clone
2G10
Applications
ELISA, WB, IHC
Field of Research
Immunology, Cancer, Developmental biology, Neuroscience, Stem Cells
Purification
Protein A+G purification
Dilution
WB: 1:1000-1:10000; IHC: 1:200-1:2000; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
55 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
CAS Number
9007-83-4
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