UBE2O antibody
Product Specifications
Background
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950) . Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138) . Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153) . Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950) . Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853) .
Synonyms
(E3-independent) E2 ubiquitin-conjugating enzyme|E2/E3 hybrid ubiquitin-protein ligase UBE2O|Ubiquitin carrier protein O|Ubiquitin-conjugating enzyme E2 O|Ubiquitin-conjugating enzyme E2 of 230 kDa (Ubiquitin-conjugating enzyme E2-230K) |Ubiquitin-protein ligase O|UBE2O|KIAA1734
Gene ID
63893
UniProt
Q9C0C9
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Ubiquitin-conjugating enzyme E2O
Target
UBE2O
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB
Field of Research
Epigenetics, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:200-1:2000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
141 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB
Curated Selection
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