TRIM25 antibody
Product Specifications
Background
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784) .
Synonyms
E3 ubiquitin/ISG15 ligase TRIM25|Estrogen-responsive finger protein|RING finger protein 147|RING-type E3 ubiquitin transferase|RING-type E3 ubiquitin transferase TRIM25|Tripartite motif-containing protein 25|Ubiquitin/ISG15-conjugating enzyme TRIM25|Zinc finger protein 147|TRIM25|EFP|RNF147|ZNF147
Gene ID
7706
UniProt
Q14258
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Tripartite motif-containing 25
Target
TRIM25
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IF, IP
Field of Research
Epigenetics, Immunology, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:200-1:2000; IP: 1:200-1:2000; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
71 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IF, IP
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
