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POLR2F antibody

Product Specifications

Background

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity) .

Synonyms

DNA-directed RNA polymerases I, II, and III subunit RPABC2 (RNA polymerases I, II, and III subunit ABC2) |DNA-directed RNA polymerase II subunit F|DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide|RPABC14.4 (RPB14.4) |RPB6 homolog|RPC15|POLR2F|POLRF

Gene ID

5435

UniProt

P61218

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Polymerase (RNA) II (DNA directed) polypeptide F

Target

POLR2F

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB, IF, IHC

Field of Research

Epigenetics, Metabolism

Purification

Immunogen affinity purified

Dilution

WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Molecular Weight

14 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Tested Applications

ELISA, WB, IF, IHC

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