POLR2D antibody
Product Specifications
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity) .
Synonyms
DNA-directed RNA polymerase II subunit RPB4 (RNA polymerase II subunit B4) |DNA-directed RNA polymerase II subunit D|RNA polymerase II 16 kDa subunit (RPB16) |POLR2D
Gene ID
5433
UniProt
O15514
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Polymerase (RNA) II (DNA directed) polypeptide D
Target
POLR2D
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IP
Field of Research
Epigenetics, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:2000; IP: 1:200-1:2000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
16-20 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IP
Curated Selection
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