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Phospho-Akt (Ser473) antibody

Product Specifications

Background

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification.

Synonyms

RAC-alpha serine/threonine-protein kinase|Protein kinase B (PKB) |Protein kinase B alpha (PKB alpha) |Proto-oncogene c-Akt|RAC-PK-alpha|AKT1|PKB|RAC

UniProt

P31749

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

V-akt murine thymoma viral oncogene homolog 1

Target

Phospho-Akt (Ser473)

Clonality

Monoclonal

Isotype

IgG1

Applications

ELISA, IHC, WB

Field of Research

Metabolism, Cardiovascular, Immunology, Developmental biology, Neuroscience, Stem Cells

Purification

Protein A+G purification

Dilution

WB: 1:1000-1:4000; IHC: 1:50-1:200

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Molecular Weight

60 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

5H4

Tested Applications

ELISA, IHC, WB

Immunogen Sequence

Peptide

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