PHF2 antibody
Product Specifications
Background
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
Synonyms
Lysine-specific demethylase PHF2|GRC5|PHD finger protein 2|PHF2|CENP-35|KIAA0662
Gene ID
5253
UniProt
O75151
Host
Rabbit
Reactivity
Human
Immunogen
PHD finger protein 2
Target
PHF2
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IP
Field of Research
Epigenetics, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
100 kDa, 150 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, IP
Curated Selection
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