Grin2c Antibody
Grin2c Antibody
Product Specifications
UniProt
Q00961
Reactivity
Human, Rat
Immunogen
NMDA NR2C polyclonal antibody was raised against an N-terminal fusion protein of the NR2C subunit of the NMDAR.
Target
Grin2c
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Concentration
Batch dependent
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
NMDA NR2C Subunit antibody labels the 140k NR2C band in Western blots of cerebellum, and it labels the 180k NR2A and NR2B subunits of the NMDAR. Immunolabeling blocked by preadsorption of antibody with the N-terminal fusion protein of the NMDAR2C that was used to generate the antibody. Antibody dilutions and tissue load should be based on tissue type and expected expression level of the protein. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB) . Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.
Tested Applications
IP, WB
NCBI Accession Number
Q00961
Host or Source
Rabbit
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