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Grin2c Antibody

Grin2c Antibody

Product Specifications

UniProt

Q00961

Reactivity

Human, Rat

Immunogen

NMDA NR2C polyclonal antibody was raised against an N-terminal fusion protein of the NR2C subunit of the NMDAR.

Target

Grin2c

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Concentration

Batch dependent

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

NMDA NR2C Subunit antibody labels the 140k NR2C band in Western blots of cerebellum, and it labels the 180k NR2A and NR2B subunits of the NMDAR. Immunolabeling blocked by preadsorption of antibody with the N-terminal fusion protein of the NMDAR2C that was used to generate the antibody. Antibody dilutions and tissue load should be based on tissue type and expected expression level of the protein. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB) . Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

Tested Applications

IP, WB

NCBI Accession Number

Q00961

Host or Source

Rabbit

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