NES antibody
Product Specifications
Background
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Synonyms
Nestin|NES
Gene ID
10763
UniProt
P48681
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Nestin
Target
NES
Clonality
Monoclonal
Isotype
IgG1
Applications
ELISA, WB, IHC, IF
Field of Research
Cardiovascular, Immunology, Cancer, Developmental biology, Neuroscience, Stem Cells
Purification
Protein A+G purification
Dilution
WB: 1:500-1:2000; IHC: 1:20-1:1000; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
170 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
4F9
Tested Applications
ELISA, WB, IHC, IF
Frequently Asked Questions
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