MPRIP antibody
Product Specifications
Background
Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Synonyms
Myosin phosphatase Rho-interacting protein (M-RIP) |Rho-interacting protein 3 (RIP3) |p116Rip|MPRIP|KIAA0864|MRIP|RHOIP3
Gene ID
23164
UniProt
Q6WCQ1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Myosin phosphatase Rho interacting protein
Target
MPRIP
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IF, IP
Field of Research
Signal Transduction
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
116-140 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, IF, IP
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