CTGF Antibody

Product Specifications

Product Name Alternative

CCN2, NOV2, HCS24, IGFBP8, CCN2, Connective tissue growth factor, CCN family member 2, IBP-8

UniProt

P29279

Reactivity

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.

Target

CTGF

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μg/mL), a concentration of 6.0 - 9.0 μg/mL of this antibody is required.ELISA:Indirect:To detect hCTGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF. Sandwich:To detect hCTGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required.Western Blot:To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.