MBP-Tag antibody
Product Specifications
Background
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
Synonyms
Maltose Binding Protein|MBP|MBP Tag
Host
Rabbit
Reactivity
Recombinant protein, Human
Immunogen
MBP-Tag
Target
MBP-Tag
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB
Purification
Immunogen affinity purified
Dilution
WB: 1:1000-1:6000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
40 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB
Frequently Asked Questions
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