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IL6 Antibody

IL6 Antibody

Product Specifications

Product Name Alternative

HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell stimulatory factor 2

UniProt

P05231

Reactivity

Human

Immunogen

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-6. Human IL-6 specific antibody was purified by affinity chromatography employing immobilized hIL-6 matrix.

Target

IL6

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (5 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody is required.ELISA:Indirect:To detect hIL-6 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6.SandwichTo detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-6 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot:To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, NeA, WB

NCBI Accession Number

P05231

Host or Source

Goat

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