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BMP4 Antibody

BMP4 Antibody

Product Specifications

Product Name Alternative

Bone morphogenetic protein, BMP2B, DVR4, Bone morphogenetic protein 4, Bone morphogenetic protein 2B, BMP-4, ZYME, OFC11, BMP2B1, MCOPS6

UniProt

P12644

Reactivity

Human

Immunogen

BMP-4 antibody is produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen.

Target

BMP4

Clonality

Monoclonal

Clone

3H2

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot:To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.

Tested Applications

ELISA, NeA, WB

NCBI Accession Number

P12644

Host or Source

Mouse

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