HELB antibody
Product Specifications
Background
5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed:25617833, PubMed:26774285) . Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285) . As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285) . Acts independently of TP53BP1 (PubMed:26774285) . Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates (PubMed:12181327) . During S phase, may facilitate cellular recovery from replication stress (PubMed:22194613) .
Synonyms
DNA helicase B (hDHB) |HELB
Gene ID
391723
UniProt
Q8NG08
Host
Rabbit
Reactivity
Human
Immunogen
Helicase (DNA) B
Target
HELB
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IF
Field of Research
Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:2000; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
120-140 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IF
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