WLS antibody
Product Specifications
Background
GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).
Synonyms
Protein wntless homolog|Integral membrane protein GPR177|Protein evenness interrupted homolog (EVI)|Putative NF-kappa-B-activating protein 373|WLS|C1orf139|GPR177
Gene ID
79971
UniProt
Q5T9L3
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
G protein-coupled receptor 177
Target
WLS
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IP
Field of Research
Signal Transduction
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
46-62 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
CAS Number
9007-83-4
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