GFP tag antibody
Product Specifications
Background
Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.
Synonyms
CFP|eGFP|eYFP|GFP|GFP tag|YFP
Host
Rabbit
Reactivity
Recombinant protetin, Aequorea victoria, Human
Immunogen
GFP tag
Target
GFP tag
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IP
Purification
Immunogen affinity purified
Dilution
WB: 1:1000-1:4000; IP: 1:2000-1:20000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
30 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
CAS Number
9007-83-4
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