Flag tag antibody
Product Specifications
Background
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x) DYKDDDDK peptide and detects FLAG-tagged proteins.
Host
Rabbit
Reactivity
Recombinant protein
Immunogen
Flag tag
Target
Flag tag
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IF
Purification
Immunogen affinity purified
Dilution
WB: 1:1000-1:4000; IF: 1:10-1:100
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IF
Frequently Asked Questions
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