FEN1 antibody
Product Specifications
Background
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Synonyms
Flap endonuclease 1 (FEN-1)|DNase IV|Flap structure-specific endonuclease 1|Maturation factor 1 (MF1, hFEN-1)|FEN1|RAD2
Gene ID
2237
UniProt
P39748
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
flap structure-specific endonuclease 1
Target
FEN1
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IF, IP
Field of Research
Epigenetics, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100; IP : 1:50 - 1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
42 kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
CAS Number
9007-83-4
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