EMG1 antibody
Product Specifications
Background
S-adenosyl-L-methionine-dependent pseudouridine N (1) -methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3- (3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967) . Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity) .
Synonyms
Ribosomal RNA small subunit methyltransferase NEP1|18S rRNA (pseudouridine (1248) -N1) -methyltransferase|18S rRNA Psi1248 methyltransferase|Nucleolar protein EMG1 homolog|Protein C2f|Ribosome biogenesis protein NEP1|EMG1|C2F
Gene ID
10436
UniProt
Q92979
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
EMG1 nucleolar protein homolog
Target
EMG1
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IF
Field of Research
Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:2000; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
27 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IF
Curated Selection
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