EIF5B antibody
Product Specifications
Background
Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
Synonyms
Eukaryotic translation initiation factor 5B (eIF-5B) |Translation initiation factor IF-2|EIF5B|IF2|KIAA0741
Gene ID
9669
UniProt
O60841
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Eukaryotic translation initiation factor 5B
Target
EIF5B
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IF
Field of Research
Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
175 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, IF
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