CHAF1A antibody
Product Specifications
Background
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity) .
Synonyms
Chromatin assembly factor 1 subunit A (CAF-1 subunit A) |Chromatin assembly factor I p150 subunit (CAF-I 150 kDa subunit, CAF-I p150, hp150) |CHAF1A|CAF|CAF1P150
Gene ID
10036
UniProt
Q13111
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Chromatin assembly factor 1, subunit A (p150)
Target
CHAF1A
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IHC, WB, IP, IF
Field of Research
Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
150 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, IHC, WB, IP, IF
Curated Selection
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