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BrdU antibody

Product Specifications

Background

Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Synonyms

5-bromo-2-deoxyuridine|BrDu|BrdUrd|Bromodeoxyuridine|BUdR

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

compound

Target

BrdU

Clonality

Monoclonal

Isotype

IgG2a

Clone

9E8

Applications

ELISA, IF, IHC

Field of Research

Epigenetics, Neuroscience, Cell Division and Proliferation

Purification

Protein A+G purification

Dilution

IHC: 1:200-1:1000; IF: 1:50-1:500

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)

CAS Number

9007-83-4

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