BrdU antibody
Product Specifications
Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
Synonyms
5-bromo-2-deoxyuridine|BrDu|BrdUrd|Bromodeoxyuridine|BUdR
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Compound
Target
BrdU
Clonality
Monoclonal
Isotype
IgG2a
Applications
ELISA, IF, IHC
Field of Research
Epigenetics, Neuroscience, Cell Division and Proliferation
Purification
Protein A+G purification
Dilution
IHC: 1:200-1:1000; IF: 1:50-1:500
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
9E8
Tested Applications
ELISA, IF, IHC
Immunogen Sequence
Compound
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