Alpha Tubulin antibody

Product Specifications

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Synonyms

Gene ID

10376

UniProt

P68363

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Tubulin, alpha 1b

Target

Alpha Tubulin

Clonality

Monoclonal

Isotype

IgG2b

Applications

ELISA, IHC, IF, WB, FC, IP

Field of Research

Neuroscience, Cell Division and Proliferation, Metabolism

Purification

Protein A+G purification

Dilution

WB: 1:2000-1:10000; IP: 1:500-1:5000; IF: 1:50-1:500; IHC: 1:50-1:500

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Molecular Weight

50-55 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Clone ID

4A10

Tested Applications

ELISA, IHC, IF, WB, FC, IP

Immunogen Sequence

1-451aa

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