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ADHFE1 antibody

Product Specifications

Background

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG) . D, L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.

Synonyms

Hydroxyacid-oxoacid transhydrogenase, mitochondrial (HOT) |Alcohol dehydrogenase iron-containing protein 1 (ADHFe1) |Fe-containing alcohol dehydrogenase|ADHFE1

Gene ID

137872

UniProt

Q8IWW8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Alcohol dehydrogenase, iron containing, 1

Target

ADHFE1

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB, IHC, IP

Field of Research

Metabolism

Purification

Immunogen affinity purified

Dilution

WB: 1:1000-1:10000; IHC: 1:20-1:200; IP: 1:200-1:1000

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Molecular Weight

50 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Tested Applications

ELISA, WB, IHC, IP

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