ADHFE1 antibody
Product Specifications
Background
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG) . D, L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
Synonyms
Hydroxyacid-oxoacid transhydrogenase, mitochondrial (HOT) |Alcohol dehydrogenase iron-containing protein 1 (ADHFe1) |Fe-containing alcohol dehydrogenase|ADHFE1
Gene ID
137872
UniProt
Q8IWW8
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Alcohol dehydrogenase, iron containing, 1
Target
ADHFE1
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IP
Field of Research
Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:1000-1:10000; IHC: 1:20-1:200; IP: 1:200-1:1000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
50 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, IP
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